Tracing your maternal and paternal ancestry with our ancestry combo
Your mtDNA is your link back to all of your maternal ancestors and your Y-DNA is your link back to all of your paternal ancestors. Analyze both in this simple ancestry combo kit.
mtDNA for your Maternal Ancestry
- Your mtDNA is passed down unchanged from mother to child and is the only way to accurately trace your maternal ancestry. Take this test to SEQUENCE your mtDNA and identify all of your mtDNA genetic variants.
Your mtDNA genome contains 16,569 base pairs. That is 16,569 different places that a change can occur! DNA SNP chips (used by many of our competitors) detect less than 20% of all these possible changes. We use Sanger Sequencing (the GOLD standard for DNA sequencing) to identify every single base pair. If you carry a mtDNA genetic variant, our technology will detect it, GUARANTEED.
The mtDNA genome contains three regions: two hypervariable regions (HVR1 and HVR2) and the more conserved coding region:
- Sequence just one or both of your hypervariable regions so that you can accurately compare your mtDNA to published data from hundreds of famous historical figures and ethnic groups around the world. DNA SNP chips struggle to detect all the variation in the hypervariable regions, but our Sanger Sequencing technology will identify every single change.
- Upgrade to the full mtDNA genome sequence (HVR1 + HVR2 + coding region) to confirm your ancient maternal history (mtDNA haplogroup and subclade).
Y-DNA for your Paternal Ancestry
- Your Y-DNA is passed down unchanged from father to son and is the only way to accurately trace your paternal ancestry. Take this test to analyze your fast-changing STR markers to trace your RECENT ancestry.
Your Y-DNA contains both slow and fast-changing genetic markers. DNA SNP chips (used by many of our competitors) detect slow-changing SNPs, which are excellent for tracing ancient ancestry but useless for anything recent (i.e. the last few hundred years). So if you want to use your DNA to help build your family tree, you need to look at fast-changing genetic markers – STRs. And that is exactly what our test detects – STRs found on your Y chromosome.
Our paternal ancestry test can analyze 20, 44 or 101 different Y-DNA STRs. The choice is yours, and the more you choose, the higher your resolution will be. Compare your Y-DNA to published data from hundreds of famous historical figures and ethnic groups around the world. Our test even allows two males to determine how many generations ago they shared a common ancestor.Important! This ancestry combo test can only be taken by MALES, as only males have Y-DNA. Females are able to trace their maternal lineages through the DNA Maternal Ancestry Test, but if they wish to trace their own paternal lineage, they must test the Y-DNA of a direct male line relative.
What is mtDNA?
Mitochondrial DNA (mtDNA) is the circular DNA which is found in the mitochondria of human cells. Each cell can contain several mitochondria and each mitochondrion contains dozens of copies of the 16,569 base pairs of mtDNA so in every cell there can be thousands of copies of mtDNA. This differs from only two copies per cell of the linear and much larger nuclear genome of 3.3 billion base pairs, which includes the autosomal and sex chromosomes. Each individual inherits mtDNA from only their mother, hence there is no recombination (mixing) of the mtDNA from generation to generation. This ensures that mtDNA essentially remains unchanged through the maternal line, providing an incredibly useful way to trace maternal ancestry.
What is mtDNA sequencing?
The circular mitochondrial genome has three regions – two small hypervariable (HVR) regions and a large coding region. Most of the variation in the mtDNA occurs in the non-coding HVR regions, as this variation does not affect the function of any proteins etc. The mtDNA sequencing available here can sequence just the HVR1 region, both hypervariable regions (HVR1 and HVR2) or the complete mitochondrial genome (HVR1, HVR2 and the coding region). HVR1 contains approximately 500 base pairs and HVR2 is approximately 400 base pairs. If two individuals have a perfect match at their HVR1 and HVR2 regions, further comparison of the much larger coding region can provide a higher stringency comparison and further resolution. The coding region covers the remainder of the mitochondrial genome and the complete mitochondrial genome is 16,569 base pairs.
Methods and analysis of mtDNA sequencing:
The mtDNA sequencing analysis uses a technique called Sanger sequencing to determine the DNA sequence in the specified region of the mtDNA. The entire DNA sequence for each region tested is provided to you in your mtDNA test report. If you choose to test all three regions (HVR1, HVR2 and coding region), you will receive a reading on all 16,569 base pairs of your mtDNA. If only one or both hypervariable regions are sequenced, a shorter reading will be provided. Your mtDNA sequencing results are also compared to a reference sequence called “rCRS” (revised Cambridge Reference Sequence) and all of the positions within your mtDNA which differ from rCRS are listed in your report.
Your unique mtDNA sequence result is known as your mtDNA profile. Individuals share the same mtDNA profile if their mtDNA sequences are an exact match to each other. Since mtDNA is passed down from mother to child along the direct maternal lineage, individuals who have descended from the same maternal lineage are expected to have exactly the same or very similar mtDNA profiles. If two individuals have different mtDNA profiles, it would conclusively confirm that they did not descend from the same maternal lineage, regardless of family legend.
What is Y-DNA?
Y-DNA refers to the DNA on the male sex chromosome – the Y chromosome. We each have 23 pairs of chromosomes: 22 pairs of autosomal chromosomes and two sex chromosomes – the X and Y chromosomes. Females inherit an X chromosomes from each parent (but no Y chromosome), while males inherit an X chromosome from their mother and a Y chromosome from their father. Very little recombination (mixing) occurs between the X and Y chromosomes in males, hence the Y-DNA essentially remains unchanged through the paternal line, providing an incredibly useful way to trace paternal ancestry.
What is the Y-DNA STR test?
The Y-DNA STR test is a useful technique used in ancestry, relationship and forensic applications. The test analyzes markers in the Y chromosome known as STRs or ‘short tandem repeats’. STR markers are short segments of DNA (2-13 nucleotides in length), which are repeated multiple times. STR analyses measure the exact number of repeat units. The number of repeats differs between individuals because STRs change frequently. The more closely related two individuals are along their direct paternal line, the more similar their Y-DNA STR profiles will be. Y-DNA STR tests can be used for forensic applications, investigating relationships, tracing distant male relatives who may have descended from the same paternal lineage and identifying links to famous people. It is a simple test that can provide you with rich information about your paternal lineage.
Methods and analysis of Y-DNA STR testing:
The Y-DNA STR Marker test uses a technique called polymerase chain reaction (PCR) to examine a defined set of STR markers in your Y-DNA. This technique determines the number of repeats at each marker tested – either 20, 44 or 101 markers, depending on the requested analysis.
When two individuals take the Y-DNA STR marker test, their STR numbers can be compared to see if there is a match. The confidence with which the Y-DNA STR test can predict a relationship between two individuals increases as more STR markers are tested. A calculation called “TMRCA” (time to most recent common ancestor) can be performed to estimate how many generations ago the two males likely shared a common paternal ancestor. Comparing more Y-DNA STR markers will provide a higher stringency comparison and a more precise TMRCA calculation.
Customers can select which mtDNA region(s) and number of Y-DNA STR markers they would like to have analyzed in this ancestry combo:
- Standard Ancestry Combo Test: 20 Y-DNA STR markers and mtDNA HVR1 sequence. This is often all that is required to achieve an adequate resolution.
- Advanced Ancestry Combo Test: 44 Y-DNA STR markers and mtDNA HVR1 + HVR2 sequence.
- Premium Ancestry Combo Test: 101 Y-DNA STR markers and the full mtDNA genome sequence (HVR1 + HVR2 + Coding Region). This provides the highest resolution.
- Upgrade Options: A cost-efficient upgrade option is available, where customers can initially select just the Standard Ancestry Combo Test. If this region does not provide a high enough resolution, there is an option to upgrade to the Advanced Ancestry Combo Test or Premium Ancestry Combo Test.
Three Easy Steps
Step 1: Order test kit online
Step 2: Collect DNA sample using a painless mouth swab, and mail to the lab in the provided return envelope
Step 3: Receive your results online
Click here for more details and to watch our explanatory video.
|✓ Define your maternal and paternal ethnic background|
|✓ Understand your maternal and paternal origins|
|✓ Search a global database for long-lost relatives on your maternal and paternal lines|
|✓ Compare your mtDNA and Y-DNA against famous people throughout history, including famous and infamous individuals, royalty, outlaws, world leaders, poets, and more|
|✓ Painless buccal (mouth) swab sample collection|
|✓ All tests are run 2x for absolute accuracy|
|✓ Quickly collect samples in the privacy of your own home|
|✓ 24/7 online test status check|
|✓ No age limit|
|✓ State-of-the-art accredited testing laboratory|